2024 Sagecreation minichemi

Sagecreation minichemi

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WebFeb 1, 2000 · A conventional image analysis was performed to determine the relative level of α-tubulin by Western blotting and IC assay. 24 IC assay has been usually used as a … WebJul 3, 2024 · All-trans retinoic acid (atRA), which is mainly generated endogenously via two steps of oxidation from vitamin A (retinol), plays an indispensible role in the development of the kidney and many other organs. Enzymes that catalyze the oxidation of retinol to generate atRA, including aldehyde dehydrogenase 1 family (ALDH1)A1, ALDH1A2 and ALDH1A3, …

MiniChemi™迷你型化学发光成像仪-北京赛智创业科技有限公司

http://bio-river.com/wp/?page_id=291 WebMar 15, 2024 · After washing three times, the membrane was stained with Immobilon Western Chemiluminescent HRP Substrate (Millipore) and examined using Minichemi … henderson county jail ky phone number

Sage Creation Sciences China – BIORIVER

WebDec 15, 2024 · All antibodies came from Cell Signaling Technology. Detection was performed with ECL substrate (Thermo Scientific, cat. # 32109). Digital images were taken by a MiniChemi from SAGECREATION, Beijing, China. Statistical analysis. Statistical analysis was conducted by using GraphPad Prism (Version 6.0). Data represent the mean ± SEM. WebMay 17, 2024 · After washing each membrane with TBST (Applygen, Beijing, China) several times, Immobilon Western Chemiluminescent HRP substrate (Millipore, MA, USA) was added, and the blots were analyzed using a MiniChemi chemiluminescence 610 imaging system (Sage Creation, Beijing, China). Protein expression was normalized to β-actin levels. http://www.sagecreation.com.cn/ lansing heating burnham ave

Antiviral activities of sea perch type I and type II ... - ScienceDirect

NAP1L1 promotes tumor proliferation through HDGF/C-JUN

WebMar 3, 2024 · Blots were developed with the detection reagents (EMD Millipore, Darmstadt, Germany), and quantified using a gel documentation and image-analysis software (MiniChemi, SageCreation, Beijing, China ... Webtive second antibody at room temperature. Signals were detected using MiniChemi Imager (SageCreation, Beijing, China). β-actin was used as the endogenous control. TP determination Cellular ATP levels were determined by ATP kit following the manufacturer’s protocol (Beyotime, China). In brief, henderson county jail kentucky inmate locatorWebAfter blocking with 5% non-fat milk, the membranes were incubated with primary antibodies overnight at 4°C. The next day, the membranes were labelled with secondary antibody. Signals were detected using MiniChemi Imager (SageCreation, Beijing, China). β-actin was used as the endogenous control. Cell viability assay henderson county jail kentucky inmate search

"WebCat.No.29050) on MiniChemi™ (SageCreation) instrument. Quantification of band intensities was processing using Lane 1D 5.0. Cell viability assay In cell viability experiments, cells were suspended in 50 μL of culture medium in 96-well cell culture plate (5000 cells/well). The stock compound in DMSO was diluted with 50 μL of culture medium, " - Sagecreation minichemi

Sagecreation minichemi

Structural basis for Sarbecovirus ORF6 mediated blockage of

WebMar 16, 2024 · Cytochrome P450 26A1 (CYP26A1) plays a vital role in early pregnancy in mice. Our previous studies have found that CYP26A1 affects embryo implantation by modulating natural killer (NK) cells, and that there is a novel population of CYP26A1 + NK cells in the uteri of pregnant mice. The aim of this study was to investigate the effects of … WebMar 1, 2024 · After being sealed with 5% skim milk powder for 1 h, the antibody of the target protein was added and incubated at 4 °C overnight. Finally, after cleaning the PVDF membrane, the second antibody was incubated for 1 h. A Chemiluminescence imaging analysis system was used for protein detection (MiniChemi 610 plus, Sagecreation, China).

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WebBlots were developed using Tanon™ High-sig ECL Western Blotting Substrate and imaged with a MiniChemi (SAGECREATION) imaging system. RNA Extraction and Real-Time qPCR. Total RNA was extracted from samples (RNAprep pure Cell/Bacteria Kit; TIANGEN) and quantified using a Nanodrop 2000 spectrophotometer prior to storage at -80°C. Webthe Minichemi ™ Chemiluminescence Imaging System (Sagecreation, BJ, China). Western blotting Western blotting was conducted as previously described [9]. e antibodies (Supplementary Table S3) used were anti-GAPDH(pAb AP0063), NAP1L1(mAb ab178687), hepatoma-derived growth factor (HDGF)(pAb 11,344– 1-AP), jun proto-oncogene (c …

WebSensor Cooling Temperatures. - 68 °C. General. Dimensions. 330x330 x 550 mm. Software. Internal SageCapture™ imaging software, Lane 1D™ data analysis software free download. High Sensitivity Imaging System. A 6 megapixel, deeply cooled (-68°C) scientific-grade CCD camera and F0.95/25mm wide aperture lens captures weak chemiluminescence ... WebMar 2, 2024 · After washing the membranes with PBS, the secondary antibody (goat anti-rabbit IgG(H + L) HRP, 1:1000, Affinity Biosciences) was added and incubated at 37 °C. …

http://sagecreation.com.cn/?c=show&catid=18 WebJan 8, 2024 · Background. Benign prostate hyperplasia (BPH) is one of the most prevalent diseases in aging males with lower urinary tract symptoms. BPH was identified as a disease after the nineteenth century and has distinctive proliferation features through the prostate transitional zone of stromal and epithelial cells.

WebMar 23, 2015 · The protein bands were visualized with enhanced chemiluminescence prime western blotting detection reagent (Amersham, GE Healthcare, Shanghai, China) and images were captured using the MiniChemi professional machine (SageCreation Science, Co., Ltd., Beijing, China). Statistical analysis

WebAug 6, 2024 · 1. Introduction. SPARC (osteonectin or BM-40), as a member of the secreted protein acidic and rich in cysteine (SPARC) family of acidic secretory glycoprotein, is known in a variety of cell functions, including cell proliferation, cell turnover, cell differentiation and tissue repair [1,2,3].SPARC has been reported to play important roles in skeletal muscle … henderson county jail mugshotsWebAug 15, 2024 · To gain structural insight into Sarbecovirus ORF6-mediated nuclear transport inhibition and innate immunity suppression, we sought to determine the crystal structure … henderson county jail inmates txWebDeer antlers are the only known mammalian organ with vascularized cartilage that can completely regenerate. Antlers are of real significance as a model of mammalian stem cell-based regeneration with particular relevance to the fields of henderson county jail lookupWebFeb 7, 2024 · Bands are obtained by Western Blotting Substrate (32106. Pierce™ ECL Western Blotting Substrate, Thermo Scientific, USA), and captured by chemiluminescence imaging systems (MiniChemi™ I, SAGECREATION, china). Co-immunoprecipitation. MDA231, MCF7 and HEK293T cells were co-transfected with BRD7 and YB1 expression … henderson county jail inmates texasWebFeb 23, 2024 · Pyropia yezoensis, belonging to the Rhodophyta, is an economically important seaweed. In this study, we developed a high-efficiency plastid transformation platform for P. yezoensis. In the plastid transformation vector, psbA UTR of P. yezoensis, including the promoter and 3′ UTR, was used to express foreign genes. The integration site was a … henderson county jail ky inmatesWeb结构更简单，系统稳定性更高！. 整体仪器外形十分精巧，节约空间. 整体仪器宽度仅41CM，. 再也不用担心有空间不足的烦恼了。. 全新电容式触摸屏. 支持多点触控，操作更加直观. … henderson county jail north carolinaWebFeb 14, 2024 · BRD7 functions as a crucial tumor suppressor in numerous malignancies including nasopharyngeal carcinoma (NPC). However, its function and exact mechanisms involved in tumor progression are not ... henderson county jail jobs