Webunique property of the photocleavable (PC) linker has promoted its application as a tool for separating, purifying, and identifying desired target biomolecules (6–8). The PC spacer phosphora-midite provides a potentially convenient tool for attachment of a wide range of functional groups to DNA by using solid-phase synthesis chemistry (8, 9). Photo-cleavable (PC) modifications contain a photolabile functional group that is cleavable by UV light of specific wavelength (300–350 nm). The PC Spacer is a 10-atom linker arm that can only be cleaved when exposed to UV light within the appropriate spectral range. The resulting oligo will have a 5’ phosphate group that is available for ...
PC Spacer (photocleavable) Oligo Modifications from Gene Link
WebUV-MALDI analysis reveals the result of the hybridization experiment along with the peptide fragments as photocleavable mass markers (PCMM). 7 By incorporating a … WebPhotocleavable DBCO-NHS ester reagent contains an amine reactive group linked to DBCO moiety through a spacer arm containing a photocleavable moiety. Molecules jointed by the copper-free click reaction can be efficiently photoreleased, typically >90% in 5-25 minutes using an inexpensive, near-UV, low intensity lamp (e.g. 365 nm lamp at 1-5 mW ... renata zanin
Photocleavable PC Linker - biosyn.com
WebPC Spacer (photocleavable) is a non-nucleosidic molety that can be used as an intermediary to attach any available phosphoramidite modification at either end of an oligonucleotide … WebApr 22, 2024 · imparted by the insertion of the photocleavable spacer could allow more orientational freedom for biotin, resulting in a higher number of detected avidin − biotin interactions. WebBio-Synthesis offers photocleavable PC Biotin Oligo Modification at various scales. This light activatable PC biotin modified oligonucleotide contained a photocleavable spacer between the biotin group and the DNA bases. The biotin group is separated from the 5' end nucleotide base by the photo-cleavable group and a long chain spacer arm. renata zamida