Pdgfb-icreert2
SpletThe Tg (Pdgfb-icre/ERT2) 1Frut allele was created by insertion of Cre/ERT2 coding cDNA at the start codon of the Pdgfb gene in a PAC, which was … Splet10. mar. 2024 · Both utilized endothelial-specific lineage tracing mice to determine the transcriptomes of endothelial cells and their progeny using Pdgfb-iCreERT2 35 or Cdh5-CreERT2 (VE-cadherin) lines. 36 Both studies found clusters primarily populated by endothelial cells in post-infarct mice, which maintained genes associated with …
Pdgfb-icreert2
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Splet029684 - Pdgfr-β-CreER [T2] Strain Details. Technical Information Scientists. Overview. Splet07. mar. 2024 · Tg (Pdgfb-icre/ERT2,-EGFP)1Frut. Mutation details : The mouse Pdgfb promoter was used to drive inducible expression of an improved cre gene fused to ERT2 …
SpletFumiaki Uchiumi, in Bidirectional Gene Promoters, 2024. Growth factors and receptors. The PDGFB (c-sis) [12] and CSF1R (c-fms) [13] are the earliest identified proto-oncogenes, … Splet27. okt. 2008 · For β-cat deletion, in vivo Pdgfb-iCreERT2 (Claxton et al., 2008) lines were crossed to mice harboring floxed alleles of β-cat (β-cat lox/lox; Brault et al., 2001) to obtain homozygous floxed β-cat lox/lox pups, of which 50% also carried one Pdgfb-iCreERT2 allele. 4OHT (Sigma-Aldrich) was dissolved in absolute ethanol at 20 mg/ml, diluted ...
Splet029684 - Pdgfr-β-CreER [T2] Strain Details. Technical Information Scientists. Overview.
Splet27. maj 2024 · On postnatal day 3, mice were administered 50 μg of 4-hydroxi-tamoxifen (H7904, Sigma-Aldrich) by intragastric injection to induce genetic inactivation of the endothelial Pdcd10 gene in littermates with Pdgfb-iCreERT2 (Pdcd10 ECKO), and Pdcd10 fl/fl mice were used as littermate controls.
Splet19. jun. 2024 · iCreERT2 activity was induced using a single intraperitoneal injection of 150 mg/ kg tamoxifen in 200 μl peanut oil resulting in Brainbow2.1 transgene (RFP, nuclear … fda list of 483sSpletPdgfb-CreER mice. Note: supply of these mice from University College London will include one male and two females as a package. Only one package will be supplied for each … frog and toad daycare essexSplet11. jun. 2024 · The vasculature of PDGFB-iCreERT2 KRIT1 ECKO mice is extremely delicate, and extra care should be taken to avoid rupturing any vessels. Excessive blood loss into the superfusate should be considered an imaging failure. 15. A number of fluorescent tracer molecules have been used for intravital microscopy. We use 10% BSA in PSS to … frog and toad coral bay paphos cyprusSplet(D) The ratio between soluble P-selectin concentration and platelet count is significantly increased in Pdgfb-iCreERT2;JAK2 V617F/WT mice. Results are expressed as mean … frog and toad desktop wallpaperSplet09. nov. 2024 · We tested administration of a monoclonal antibody that enforces the active β1 integrin conformation, (clone 9EG7), on a murine neonatal CCM mouse model, Krit1 flox/flox;Pdgfb-iCreERT2 (Krit1 ECKO), and on KRIT1-silenced human umbilical vein endothelial cells (HUVECs). frog and toad complete collectionSplet05. feb. 2024 · To gain insight into the nuclear function of YAP and TAZ, we generated a Pdgfb-iCreERT2-inducible TAZ gain-of-function mouse allele, in which a mutated version of TAZ (TAZ S89A) is introduced in the Rosa26 locus and expressed by a CAG promoter following Cre-mediated excision of an upstream stop cassette (Figure 7—figure … fda list of nti drugsSplet27. okt. 2008 · For β-cat deletion, in vivo Pdgfb-iCreERT2 (Claxton et al., 2008) lines were crossed to mice harboring floxed alleles of β-cat (β-cat lox/lox; Brault et al., 2001) to obtain homozygous floxed β-cat lox/lox pups, of which 50% also carried one Pdgfb-iCreERT2 allele. 4OHT (Sigma-Aldrich) was dissolved in absolute ethanol at 20 mg/ml, diluted ... frog and toad crosses