2024 Germacrene synthase

Germacrene synthase

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August undefined, 2024

WebDespite their importance, only four terpene synthases have been identified from the Zingiberaceae family: (+)-germacrene D synthase and (S)-β-bisabolene synthase from ginger rhizome, and α-humulene synthase and β-eudesmol synthase from shampoo ginger (Zingiber zerumbet) rhizome. We report the identification of 25 mono- and 18 … WebAug 11, 2024 · Germacrene A (GA) is a key intermediate for the synthesis of medicinal active compounds, especially for β-elemene, which is a broad-spectrum anticancer drug. …

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WebDec 17, 2015 · Germacrene A synthase (GAS) from Solidago canadensis catalyzes the conversion of farnesyl diphosphate (FDP) to the plant sesquiterpene (+)-germacrene A. After diphosphate expulsion, farnesyl cation reacts with the distal 10,11-double bond to afford germacrene A (>96%) and 2% α-humulene, which arises from 1,11-cyclization of … WebJan 7, 2024 · The sesquiterpene germacrene A is a direct precursor of ß-elemene that is a major component of the Chinese medicinal herb Curcuma wenyujin with prominent … perler bead arcade phone holder

Germacrene A Biosynthesis Plant Physiology Oxford Academic

WebJun 26, 2024 · Zager and colleagues reported the identities of CsTPS16, a germacrene B synthase, and CsTPS20, a hedycaryol synthase. We were able to confirm the activities of both of these enzymes as germacrene B and hedycaryol synthases, respectively, using CsTPS16 and CsTPS20 cloned from cv PK [CsTPS16(PK) and CsTPS20(PK), … Web'Germacrene-A synthase' published in 'Class 4–6 Lyases, Isomerases, Ligases' Keywords. Escherichia Coli; Molecular Weight; Catalytic Activity; Functional Analysis WebApr 30, 2024 · As expected, purified geosmin synthase yielded the sesquiterpenoids geosmin, germacradienol and germacrene D in the assay mixture upon incubation with FPP (S2 Fig and S3 Fig), whereas the monoterpene limonene was formed when GPP was added to limonene synthase containing crude extracts (S4 Fig and S5 Fig). perler bead animal crossing

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WebGermacrene D Receptor Neurons. One type of receptor neurons, tuned to the sesquiterpene germacrene D, frequently occurred in recordings from heliothine moths, and was the first type identified in these species57 ( Fig. 10.1 ). The response was elicited by mixtures obtained from many plant species, both hosts and non-hosts. WebJul 1, 2006 · The N-part of geosmin synthase catalyzes the cyclization FPP to germacradienol and germacrene D the C-part catalyzes cyclization and fragmentation of germacradienol into geosmin (Giglio et al ... perler bead 3d house templateWebMay 18, 2024 · Germacrene D was derived from the linear C15 precursor farnesyl diphosphate (FPP) through highly complex reactions catalyzed by germacrene D … perler bead art christmas

"WebJul 28, 2012 · A putative gene encoding germacrene D synthase (GenBank Accession No. JQ247185) from tea plant (Camellia sinensis) was isolated by rapid amplification of … " - Germacrene synthase

Germacrene synthase

WebMay 1, 2002 · de Kraker et al. (1998, 2001, 2002) showed that the sesquiterpene lactones in chicory and probably also in a large number of other plant species originate from a … WebGermacrene D Receptor Neurons. One type of receptor neurons, tuned to the sesquiterpene germacrene D, frequently occurred in recordings from heliothine moths, …

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WebApr 1, 1999 · Formation of (-)-germacrene D involves a stereospecific 1,3-hydride shift of H-1si of farnesyl diphosphate. Both products are formed from a common intermediate. The enzyme mediates a key step in the biosynthesis of geosmin (see EC 4.1.99.16), a widely occurring metabolite of many streptomycetes, bacteria and fungi. WebMay 1, 2002 · de Kraker et al. (1998, 2001, 2002) showed that the sesquiterpene lactones in chicory and probably also in a large number of other plant species originate from a common germacrane precursor, (+)-germacrene A.The biosynthesis of this sesquiterpene olefin from the ubiquitous sesquiterpene precursor farnesyl diphosphate (FDP) is catalyzed by a (+) …

WebSep 20, 2012 · Highlights This manuscript is firstly characterized for germacrene A synthase gene in Citrus species. The gene identification of mono- or sesqui-terpene …

WebOct 24, 2024 · a germacrene synthase from dandelion. Subsequently, biosynthesis of signiﬁcant amounts of. germacrene-A was observed in the most productive strain compared to the wild type. Thus, • Adio, A. M. (2009). "Germacrenes A–E and related compounds: thermal, photochemical and acid induced transannular cyclizations". Tetrahedron. 65 (8): 1533–1552. doi:10.1016/j.tet.2008.11.050. • Deguerry, F.; Pastore, L.; Wu, S.; Clark, A.; Chappell, J.; Schalk, M. (2006-10-15). "The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated with a limited number of sesquiterpene synthases". Archives of Biochemistry and Biophysics. 454 (2): 123–136. doi:10.1…

WebMar 3, 2015 · Germacrene A synthase is a two-domain, α−β-terpenoid synthase with the active center in α-domain (C-terminus, 234–558 AA) exhibiting class I terpene synthase activity (Christianson, 2008; Rajabi et al., 2013). β-domain in N-terminus (32–245 AA) has lost the catalytic activity in mono- and sesquiterpene synthases, and seems to play a ...

WebJul 16, 2024 · The genes underlying STL biosynthesis have been partially characterized. Genes encoding the enzyme catalysing the first step of the STL production, germacrene … perler bead axWebNeither germacrene A nor any other intermediate is released from sesquiterpene synthase active sites during normal catalysis. Evidence to support the intermediacy of germacrene A has been derived from investigations of aristolochene synthases from Aspergillus terreus and Penicillium roquefortii (Cane, D. E. Chem. Rev. 1990, 90, 1089-1103 and ... perler bead animals cuteWebJul 5, 2004 · --germacrene D synthase: BLAST Add. Sequence: Expand table. Expression. Tissue specificity. Expressed in flowers. Detected in stems, young leaves and tendrils. Developmental stage. Expressed in flower buds and detected in open pre-anthesis flowers, flowers after anthesis and in early stages of fruit onset. perler bead astronaut patternWebMar 3, 1998 · The germacrene synthase activity expressed from clone pLE11.3 exceeded the limonene synthase activity by a factor of 10, when measured in the same enzyme … perler bead animals easyWebMay 22, 2024 · Germacrene A synthase (GAS) catalyses a 1,10-cyclisation of FPP to B, followed by deprotonation to 1 (Scheme 3). Both enantiomers of 1 are accessible through this reaction, depending on whether C10 of … perler bead beach sunset patternsWebGermacrene A is next oxygenated by cytochrome P450 enzymes germacrene A oxidase (GAO) and costunolide synthase (COS) to form costunolide (Liu et al., 2011). Another key step to convert costunolide to the guaianolide precursor kauniolide in chicory was recently characterized, and it involves the action of three kauniolide synthases (KLS ... perler bead art ironing sheet alternativesWebAug 1, 1998 · The biosynthesis of germacrene C by a homogenate of immature seeds of Kadsura japonica has been reported (Morikawa et al., 1971), as well as the partial … perler bead axolotl