WebNov 7, 2016 · In vivo experiments. Cell suspensions were prepared at 5 x 10 3, 2 x 10 4, 5 x 10 5 and 10 x 10 6 cells/mL in phosphate-buffered solution, and maintained at room temperature. Five to six weeks old Balb/c female mice (Balb/cAnNCrl) were orthotopically inoculated within 40 min after preparation of cell suspension, in the fourth inguinal … WebFurther immunoblot assays confirmed that MIEN1 was knocked down in the xenograft tumors by which PC_shMIEN1 cells were inoculated (Figure 3I). Collectively, knockdown of MIEN1 attenuated the cell proliferation and tumorigenesis of prostate carcinoma cells. 2.4. Ectopic Overexpression of MIEN1 Attenuates Cisplatin-Induced Cell Apoptosis in …
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WebMice inoculated with B16 melanoma cells exposed to long-term in vitro IFN-alpha treatment (> or = 14 days, B16 alpha cells) but not short-term in vitro IFN-alpha treatment (24 h) exhibited an enhanced survival time. Enhanced survival time also occurred when inactivated B16 alpha cells were inoculated at the same time as live B16 cells. WebJan 31, 2024 · Briefly, confluent monolayers of MDCK cells in 12-well plates were inoculated with 200 µl of viral or sample dilutions and incubated for 1 h. The inoculum was removed then the cells were overlaid with 0.6% agarose (Oxoid) in MEM including 1 µg/ml trypsin and incubated at 37°C with 5% CO 2. After 3 days, the agarose was removed and … python 正则 json
Inoculation - Wikipedia
WebJun 17, 2024 · Medical researcher Jonas Salk created a polio vaccine that, when injected, stimulated the immune system to make antibodies that fought off the virus. By January of that year, he had inoculated 161 ... WebJan 22, 2024 · Wells were inoculated with 2 μL diluted cell suspension, resulting in a starting cell density of approx. 10 5 CFU/mL. The plate was incubated with transparent lid for 1 h at 37°C in the plate reader and luminescence was measured every 2 min from top. Then 4 μg/mL Imipenem, 50 μg/mL Ampicillin or 50 μg/mL Polymyxin B was added from … Webinoculate: 1 v impregnate with the virus or germ of a disease in order to render immune Type of: impregnate fertilize and cause to grow v perform vaccinations or produce immunity in by inoculation Synonyms: immunise , immunize , vaccinate Type of: inject , shoot give an injection to v introduce a microorganism into Types: seed inoculate with ... python 求